Chromosome-scale and haplotype-resolved genome assembly of the autotetraploid Misgurnus anguillicaudatus

Scientific Data

2024-09 | Journal article

Abstract

In nature, diploids and tetraploids are two common types of polyploid evolution. Misgurnus anguillicaudatus (mud loach) is a remarkable fish species that exhibits both diploid and tetraploid forms. However, reconstructing the four haplotypes of its autotetraploid genome remains unresolved. Here, we generated the first haplotype-resolved, chromosome-level genome of autotetraploid M. anguillicaudatus with a size of 4.76 Gb, contig N50 of 6.78 Mb, and scaffold N50 of 44.11 Mb. We identified approximately 2.9 Gb (61.03% of genome) of repetitive sequences and predicted 91,485 protein-coding genes. Moreover, allelic gene expression levels indicated the absence of significant dominant haplotypes within the autotetraploid loach genome. This genome will provide a valuable biological model for unraveling the mechanisms of polyploid formation and evolution, adaptation to environmental changes, and benefit for aquaculture applications and biodiversity conservation.

Cite this article

Sun B, Li Q, Mei Y, Zhang Y, Zheng Y, Huang Y, Xiao X, Zhang J, Gao J and Cao X. Chromosome-scale and haplotype-resolved genome assembly of the autotetraploid Misgurnus anguillicaudatusScientific Data, 2024, 11:1059. DOI: 10.1038/s41597-024-03891-z