Interspecies rice versus Arabidopsis thaliana protein–protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing

Journal of Integrative Plant Biology

2025-12 | Journal article

Abstract

Touch-down overlapping PCR coupled with HiFi long-read sequencing, a high-throughput method for large-scale profiling of protein-protein interactions based on stitch-PCR identified 7,726 high-confidence interactions between rice and Arabidopsis proteins by integrating a library-vs-library yeast two-hybrid strategy with optimized PCR and long-read sequencing.

Cite this article

Huang J#, Cheng Y#, Ruan J#, Liu X, Xia D, Chen Y, Fan D, Ying J, Wang Y, Tong X, Li Z, Xue D, Zhang J, Zhang J* and Hou Y*. Interspecies rice versus Arabidopsis thaliana protein–protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing. Journal of Integrative Plant Biology, 2025, in press. DOI: 10.1111/jipb.70107